pjmd 20 (Addgene inc)
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pjmd 20
Pjmd 20, supplied by Addgene inc, used in various techniques. Bioz Stars score: 88/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pjmd+20/pWZL+Neo+Myr+Flag+YES1+(Plasmid+%2320665)/pmc12617493__sciadv%2Eadz2217_sm-101-162-249
Average 88 stars, based on 2 article reviews
Pjmd 20, supplied by Addgene inc, used in various techniques. Bioz Stars score: 88/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pjmd+20/pWZL+Neo+Myr+Flag+YES1+(Plasmid+%2320665)/pmc12617493__sciadv%2Eadz2217_sm-101-162-249
Average 88 stars, based on 2 article reviews
pjmd 20 - by Bioz Stars,
2026-10
88/100 stars
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Cloning:Article Title: Ist2 promotes lipid transfer by Osh6 via its membrane tethering and lipid scramblase activities Article Snippet: Plasmids Alias Description References pRS416 CEN, URA3 Lab collection pOsh6-mCherry pAC100 pRS416-based, ADH1pr-Osh6-mCherry (16) pCS_39 pOsh6-mCherry- E170A ADH1pr-Osh6-mCherry, E170A; Mutations inserted by Gibson cloning from a pGex4T-3 plasmid expressing Osh6(E170A) into pAC100 plasmid This work pCS_41 pOsh6-mCherry3M ADH1pr-Osh6-mCherry, E170A, Q417A, T420I; Mutations inserted by Gibson cloning from a pGex4T-3 plasmid expressing Osh6(E170A/Q417A/T420I) into pAC100 plasmid This work pRS413 pRS413/pEV CEN, HIS3 Lab collection pAK75 GFP-Ist2 pUG34-based (CEN, HIS3), Ist2pr-BFP-Ist2 (22) pJMD_07 pIst2 pUG34-based (CEN, HIS3), Ist2pr-BFP-Ist2 (16) pJMD_13 pIst2[1–877] Ist2pr-BFP-Ist2[1–877]; Exclusion of [878-946] region from pAK75 recombined into pJMD_07 This work pJMD_10 pIst2[590–946] Ist2pr-BFP-Ist2[590–946]; Exclusion of [1-590] region from pAK75 recombined into BamHI/XhoI linearized pJMD_07 This work pJMD_30 pIst2[1–877]PM Ist2pr-BFP-Ist2[1–877]-CAAX; insertion of CAAX-coding motif by Gibson cloning into pJMD_07 This work pCS_45 pIst2736-743Δ Ist2pr-BFP-Ist2 [1–736; 743–946]; Site-directed mutagenesis of pJMD_07 This work pJMD_20 pIst2-short-tail Ist2pr-BFP-Ist2-[1–590; 705–762; 879–946]; Synthetic gene integrated by Gibson cloning into pJMD_07 This work pJMD_33 pIst2-shorttail+23aa Ist2pr-BFP-Ist2-[1–590; 682–762; 879–946]; Extension of 23 amino acids [682–704] by Gibson cloning into pJMD_20 This work pJMD_34 pIst2-shorttail+40aa Ist2pr-BFP-Ist2-[1–590; 665–762; 879–946]; Extension of 40 amino acids [665–704] by Gibson cloning into pJMD_20 This work pJMD_36 pIst2-very-long- tail Ist2pr-BFP-Ist2-[1–704; 590–704; 705–877; 763-946]; Duplication of [590–704] and [763–877] regions inserted by Gibson cloning into pJMD_20 This work pJMD_18 pIst2-BS-Osh6- ER Ist2pr-BFP-Ist2-[1–589; 705–762; 590–704; 763–946]; Position change of [705–762] region inserted by Gibson cloning into pJMD_20 This work pJMD_19 pIst2-BS-Osh6- PM Ist2pr-BFP-Ist2-[1–704; 763–877; 705–762; 878–946]; Position change of [705–762] region inserted by Gibson cloning into pJMD_20 This work LactC2-GFP pC2lact-GFP GPDprC2Lact-GFP, CEN, URA3, Plasmid Preparation:Article Title: Ist2 promotes lipid transfer by Osh6 via its membrane tethering and lipid scramblase activities Article Snippet: Plasmids Alias Description References pRS416 CEN, URA3 Lab collection pOsh6-mCherry pAC100 pRS416-based, ADH1pr-Osh6-mCherry (16) pCS_39 pOsh6-mCherry- E170A ADH1pr-Osh6-mCherry, E170A; Mutations inserted by Gibson cloning from a pGex4T-3 plasmid expressing Osh6(E170A) into pAC100 plasmid This work pCS_41 pOsh6-mCherry3M ADH1pr-Osh6-mCherry, E170A, Q417A, T420I; Mutations inserted by Gibson cloning from a pGex4T-3 plasmid expressing Osh6(E170A/Q417A/T420I) into pAC100 plasmid This work pRS413 pRS413/pEV CEN, HIS3 Lab collection pAK75 GFP-Ist2 pUG34-based (CEN, HIS3), Ist2pr-BFP-Ist2 (22) pJMD_07 pIst2 pUG34-based (CEN, HIS3), Ist2pr-BFP-Ist2 (16) pJMD_13 pIst2[1–877] Ist2pr-BFP-Ist2[1–877]; Exclusion of [878-946] region from pAK75 recombined into pJMD_07 This work pJMD_10 pIst2[590–946] Ist2pr-BFP-Ist2[590–946]; Exclusion of [1-590] region from pAK75 recombined into BamHI/XhoI linearized pJMD_07 This work pJMD_30 pIst2[1–877]PM Ist2pr-BFP-Ist2[1–877]-CAAX; insertion of CAAX-coding motif by Gibson cloning into pJMD_07 This work pCS_45 pIst2736-743Δ Ist2pr-BFP-Ist2 [1–736; 743–946]; Site-directed mutagenesis of pJMD_07 This work pJMD_20 pIst2-short-tail Ist2pr-BFP-Ist2-[1–590; 705–762; 879–946]; Synthetic gene integrated by Gibson cloning into pJMD_07 This work pJMD_33 pIst2-shorttail+23aa Ist2pr-BFP-Ist2-[1–590; 682–762; 879–946]; Extension of 23 amino acids [682–704] by Gibson cloning into pJMD_20 This work pJMD_34 pIst2-shorttail+40aa Ist2pr-BFP-Ist2-[1–590; 665–762; 879–946]; Extension of 40 amino acids [665–704] by Gibson cloning into pJMD_20 This work pJMD_36 pIst2-very-long- tail Ist2pr-BFP-Ist2-[1–704; 590–704; 705–877; 763-946]; Duplication of [590–704] and [763–877] regions inserted by Gibson cloning into pJMD_20 This work pJMD_18 pIst2-BS-Osh6- ER Ist2pr-BFP-Ist2-[1–589; 705–762; 590–704; 763–946]; Position change of [705–762] region inserted by Gibson cloning into pJMD_20 This work pJMD_19 pIst2-BS-Osh6- PM Ist2pr-BFP-Ist2-[1–704; 763–877; 705–762; 878–946]; Position change of [705–762] region inserted by Gibson cloning into pJMD_20 This work LactC2-GFP pC2lact-GFP GPDprC2Lact-GFP, CEN, URA3, Expressing:Article Title: Ist2 promotes lipid transfer by Osh6 via its membrane tethering and lipid scramblase activities Article Snippet: Plasmids Alias Description References pRS416 CEN, URA3 Lab collection pOsh6-mCherry pAC100 pRS416-based, ADH1pr-Osh6-mCherry (16) pCS_39 pOsh6-mCherry- E170A ADH1pr-Osh6-mCherry, E170A; Mutations inserted by Gibson cloning from a pGex4T-3 plasmid expressing Osh6(E170A) into pAC100 plasmid This work pCS_41 pOsh6-mCherry3M ADH1pr-Osh6-mCherry, E170A, Q417A, T420I; Mutations inserted by Gibson cloning from a pGex4T-3 plasmid expressing Osh6(E170A/Q417A/T420I) into pAC100 plasmid This work pRS413 pRS413/pEV CEN, HIS3 Lab collection pAK75 GFP-Ist2 pUG34-based (CEN, HIS3), Ist2pr-BFP-Ist2 (22) pJMD_07 pIst2 pUG34-based (CEN, HIS3), Ist2pr-BFP-Ist2 (16) pJMD_13 pIst2[1–877] Ist2pr-BFP-Ist2[1–877]; Exclusion of [878-946] region from pAK75 recombined into pJMD_07 This work pJMD_10 pIst2[590–946] Ist2pr-BFP-Ist2[590–946]; Exclusion of [1-590] region from pAK75 recombined into BamHI/XhoI linearized pJMD_07 This work pJMD_30 pIst2[1–877]PM Ist2pr-BFP-Ist2[1–877]-CAAX; insertion of CAAX-coding motif by Gibson cloning into pJMD_07 This work pCS_45 pIst2736-743Δ Ist2pr-BFP-Ist2 [1–736; 743–946]; Site-directed mutagenesis of pJMD_07 This work pJMD_20 pIst2-short-tail Ist2pr-BFP-Ist2-[1–590; 705–762; 879–946]; Synthetic gene integrated by Gibson cloning into pJMD_07 This work pJMD_33 pIst2-shorttail+23aa Ist2pr-BFP-Ist2-[1–590; 682–762; 879–946]; Extension of 23 amino acids [682–704] by Gibson cloning into pJMD_20 This work pJMD_34 pIst2-shorttail+40aa Ist2pr-BFP-Ist2-[1–590; 665–762; 879–946]; Extension of 40 amino acids [665–704] by Gibson cloning into pJMD_20 This work pJMD_36 pIst2-very-long- tail Ist2pr-BFP-Ist2-[1–704; 590–704; 705–877; 763-946]; Duplication of [590–704] and [763–877] regions inserted by Gibson cloning into pJMD_20 This work pJMD_18 pIst2-BS-Osh6- ER Ist2pr-BFP-Ist2-[1–589; 705–762; 590–704; 763–946]; Position change of [705–762] region inserted by Gibson cloning into pJMD_20 This work pJMD_19 pIst2-BS-Osh6- PM Ist2pr-BFP-Ist2-[1–704; 763–877; 705–762; 878–946]; Position change of [705–762] region inserted by Gibson cloning into pJMD_20 This work LactC2-GFP pC2lact-GFP GPDprC2Lact-GFP, CEN, URA3, Mutagenesis:Article Title: Ist2 promotes lipid transfer by Osh6 via its membrane tethering and lipid scramblase activities Article Snippet: Plasmids Alias Description References pRS416 CEN, URA3 Lab collection pOsh6-mCherry pAC100 pRS416-based, ADH1pr-Osh6-mCherry (16) pCS_39 pOsh6-mCherry- E170A ADH1pr-Osh6-mCherry, E170A; Mutations inserted by Gibson cloning from a pGex4T-3 plasmid expressing Osh6(E170A) into pAC100 plasmid This work pCS_41 pOsh6-mCherry3M ADH1pr-Osh6-mCherry, E170A, Q417A, T420I; Mutations inserted by Gibson cloning from a pGex4T-3 plasmid expressing Osh6(E170A/Q417A/T420I) into pAC100 plasmid This work pRS413 pRS413/pEV CEN, HIS3 Lab collection pAK75 GFP-Ist2 pUG34-based (CEN, HIS3), Ist2pr-BFP-Ist2 (22) pJMD_07 pIst2 pUG34-based (CEN, HIS3), Ist2pr-BFP-Ist2 (16) pJMD_13 pIst2[1–877] Ist2pr-BFP-Ist2[1–877]; Exclusion of [878-946] region from pAK75 recombined into pJMD_07 This work pJMD_10 pIst2[590–946] Ist2pr-BFP-Ist2[590–946]; Exclusion of [1-590] region from pAK75 recombined into BamHI/XhoI linearized pJMD_07 This work pJMD_30 pIst2[1–877]PM Ist2pr-BFP-Ist2[1–877]-CAAX; insertion of CAAX-coding motif by Gibson cloning into pJMD_07 This work pCS_45 pIst2736-743Δ Ist2pr-BFP-Ist2 [1–736; 743–946]; Site-directed mutagenesis of pJMD_07 This work pJMD_20 pIst2-short-tail Ist2pr-BFP-Ist2-[1–590; 705–762; 879–946]; Synthetic gene integrated by Gibson cloning into pJMD_07 This work pJMD_33 pIst2-shorttail+23aa Ist2pr-BFP-Ist2-[1–590; 682–762; 879–946]; Extension of 23 amino acids [682–704] by Gibson cloning into pJMD_20 This work pJMD_34 pIst2-shorttail+40aa Ist2pr-BFP-Ist2-[1–590; 665–762; 879–946]; Extension of 40 amino acids [665–704] by Gibson cloning into pJMD_20 This work pJMD_36 pIst2-very-long- tail Ist2pr-BFP-Ist2-[1–704; 590–704; 705–877; 763-946]; Duplication of [590–704] and [763–877] regions inserted by Gibson cloning into pJMD_20 This work pJMD_18 pIst2-BS-Osh6- ER Ist2pr-BFP-Ist2-[1–589; 705–762; 590–704; 763–946]; Position change of [705–762] region inserted by Gibson cloning into pJMD_20 This work pJMD_19 pIst2-BS-Osh6- PM Ist2pr-BFP-Ist2-[1–704; 763–877; 705–762; 878–946]; Position change of [705–762] region inserted by Gibson cloning into pJMD_20 This work LactC2-GFP pC2lact-GFP GPDprC2Lact-GFP, CEN, URA3, |